The Role of Neurotensin in Wound Healing in Diabetic and iNOS Knockout Mice
Abstract Number: 1000-P
Authors: ERMELINDO LEAL, ANA TELLECHEA, LIANE MOURA, ARISTIDIS VEVES, EUGENIA CARVALHO
Institutions: Coimbra, Portugal, Boston, MA
Results: Peripheral neuropathy impairs wound healing in diabetes while neuropeptides and inducible nitric oxide synthase (iNOS) mediate inflammatory and the healing processes. We evaluated the efficacy of neurotensin (NT) in promoting wound healing in normal, streptozotocin (STZ)-induced diabetic and iNOS knockout (iNOSKO) mice.
We studied wild-type C57BL6/J (WT), WT diabetic and iNOSKO mice. Eight weeks prior to wounding, diabetes was induced in WT mice by streptozotocin (STZ, 150 mg/kg ip). Two 6 mm excision wounds were created in the dorsum of each mouse. One wound was treated by daily applications of NT (50 μg) and the other with saline. The wound healing process was monitored up to 10 days by acetate tracing.
Neurotensin reduced the wound area at days 5, 8 and 10 post-wounding in WT mice, when compared with saline-treated wounds (*p<0.05, **p<0.01; figure 1). The effect of NT in diabetic wounds started earlier, showing an improved healing at days 3, 5, 8 and 10 (#p<0.05, ##p<0.01; figure 1).[figure1]Neurotensin reduced wound area in iNOSKO mice at day 8 (*p<0.05), and day 10 (p=0.08) (figure 2).[figure2]These results suggest that NT could be a good candidate for future therapeutic approaches in diabetic foot ulceration. Also, NT improves wound healing in iNOS knockout animals, although to a lower extent. This may suggest that NT effect can be partially due to iNOS.